Ion Channel Targets

• Voltage-gated Ion Channels
  • (K+) hERG
  • (Na+) Nav 1.5, Nav 1.7, Nav 1.1
• Ligand-gated Ion Channels
  • TRP Family (TRPV1, V3, C6)
  • P2X Family (P2X3, 4, 7)
  • GABAaR (1α, 2β, γ2)
  • GluR (AMPA, NMDA) Family

In Vitro and Ex Vivo CNS Biomarkers

• High content image analysis system for neurites
• ELISA assays of neurofilament light protein/progranulin for neuronal degeration
• Neural histology with confocal microscope, PSD95/SV2A, golgi staining
• AAV carried gene expression in cochlea and ex plant

Electrophysiology Platforms

• Transient/stable cell lines
• Primary cultural of DRG, hippocampal, cortical neurons
• Ex vivo brain slices for
  • Hippocampus (CA1, CA3, DG)
  • Frontal cortex
  • Cortex-striatum
  • N Accumbens
  • Amygdala
  • Interpeduncular nucleus
  • LTP/LTD or neuronal excitability in:
• Automated Qpatch-48
• Manual patch clamp EPC10 USB, 700B
• Multi-electrode array MED64 system